
v6.0 [Jul 14, 2015]
CodonCode Aligner version 6.0 introduces functionality for primer design and bacterial genome assembly.
Primer design in CodonCode Aligner 6 utilizes Primer3 to pick sequencing or PCR primers. Primer can be imported in the current project and are highlighted in the template sequence.
Aligner 6 add the option to create fast and memory efficient bacterial genome assemblies. Aligner's scaffolding algorithm generates accurate scaffolds with high coverage.
v5.1 [Nov 7, 2014]
CodonCode Aligner 5.1 adds functions to analyze methylation of cytosines after bisulfite modification, PCR, and capillary sequencing.
Methylation results are displayed in our features table that can be exported and used to navigate to the methylated base.
Mouse overs also show the calculated methylation ratio, as well as the intensity of the raw peaks.
Methylation analysis uses raw ABI trace data, which avoids artifacts from trace "normalization" and provides higher accuracy.